Application of enzymatic assays in the evaluation of bioproducts
Syllabus: Biochemical foundations of activity and enzymatic inhibition. Experimental models of enzymatic inhibition. Enzyme inhibition or induction assay assays and applications: proteases; amylases, glucosidases, lipases and angiotensin converting enzyme.
Legislation and Innovation
Syllabus: Discuss the Brazilian intellectual property system, patent filing and granting procedures. Assist in the writing of projects and transfer of new technologies to the industrial sector. Present successful examples of national and international agreements.
Molecular biology applied to the pharmaceutical sciences
Syllabus: The theoretical course will present an overview of the foundations of Molecular Biology. The first part will cover topics on DNA structure, replication, transcription and translation, DNA mutation and repair, protein synthesis and gene expression control. In a second part, the topics will be covered: recombinant DNA technology, protein expression studies, notions of bioinformatics, genomics and proteomics. Understanding the molecular mechanisms surrounding nucleic acids and the application of recombinant DNA technology has enabled great advances in science. New drugs, more accurate diagnostics as well as a better understanding of the molecular and biochemical mechanisms of various diseases are just some of the examples that have resulted from this new area of knowledge. Thus, the Molecular Biology course aims to provide the student with basic subsidies for the understanding and application of this knowledge in the various areas it reaches.
Writing the scientific article
Syllabus: Understanding the sequential process of writing and submitting a scientific article. Construction of the abstract. Identification of the central idea of the research. Material and method considerations, results and discussion. Reflection on the peer review process of a scientific article. Understanding the process of preparing the answers and justifications to the editor. To favor the understanding of the mechanisms involved in the elaboration and submission of a scientific article.
Syllabus: From the perspective of Medicinal Chemistry, the course addresses the concepts and definitions that characterize the three-dimensional representation of bioactive organic substances, pointing out how stereochemical and conformational factors are crucial for the complete understanding of the molecular recognition of a drug by the target bioreceptor implications associated with the therapeutic efficacy and safety of these prototypes.
Preclinical Biological Assays
Syllabus: Presentation of the course. Distribution of work groups. Introduction to Toxicology. Animal Handling and Ethics 2011-07-26 12:04 Page 18 of 179 experimentation. Introduction to toxicity assessment. Acute and subacute toxicity. Chronic toxicity. Discussion OECD guidelines for toxicity assessment (407, 408, 420, 423 and 425). Teratogenicity. Alternative methods of toxicity assessment. Mutagenicity, carcinogenicity / micronucleus. Risk analysis. Toxicity assessment seminars.
Experimental Pharmacology (Pharmacological Evaluation)
Syllabus: Basic principles of maintenance and handling of laboratory animals used in pharmacology. Dose calculation, weighing and drug dilution. Routes of drug administration. Experimental models in pharmacology (anxiety, depression, pain, inflammation, seizure, gastric ulcer, memory and learning).
Syllabus: Study of the kinetic disposition of drugs, focusing on the absorption, distribution, biotransformation and excretion aspects; study of kinetic models and their application to pharmaceutical products. Special situations and pharmacokinetics (age, diseases, gender, drug interactions).
Scientific research methodology
Summary: Study of the basic guidelines for the development of research activities with a scientific character, with an approach that will discuss the techniques, norms and rules (methods of science) that determine the quality of research and its relevance to the production of knowledge, leading to the critical question that arouses for the reflection of the proposed themes.
Molecular Modeling Applied to Drug Planning
Syllabus: The course describes the application of molecular modeling to drug planning. It introduces the computational methodologies that allow the best direction in the development process of new drugs besides the improvement of properties of existing molecules (prototypes), considering the probable interaction with the target biomachromolecule (receptor, enzyme, DNA).
Spectroscopic Identification of Organic Compounds
Syllabus: To enable the theoretical understanding and application of spectroscopic methodologies of structural elucidation of natural and synthetic compounds with potential biological activity. Assist in the interpretation of ultraviolet, infrared, mass, and one-and-two dimensional nuclear magnetic resonance (hydrogen and carbon) spectrums.
Applied Electrochemical Methods
Syllabus: History of Electrochemistry, Definitions and Classifications; Basic concepts; Basic instrumentation; General Applications of Electrochemistry and Electroanalytical; Theoretical Foundations: Basic Principles in Electrochemistry, Fundamental Equations, Voltametric Techniques, Potentiometry, Other Electrochemical Techniques: Condutometry, Impedantometry, Electrogravimetry and Condutometry, Modified Electrodes
Natural Products Chemistry
Syllabus: Biosynthesis of secondary metabolites: Photosynthesis function, Terpenes, Shikimic acid derivatives (tannins, flavonoids, coumarins, lignans), Polyacetic derivatives (anthraquinones and derivatives) and Nitrogen compounds (alkaloids). General methods of extraction, isolation and structural elucidation.
Seminar on Pharmaceutical Sciences I
Syllabus: Subjects related to the pharmaceutical sciences, relevant to the research lines of bioactive natural and synthetic products, technology and quality control. They should involve topics on bioactive compound chemistry, plant secondary metabolism, pharmacobotany, pharmacology, toxicology, pharmacokinetics, pharmacotechnics, pharmaceutical development and technology, drug quality control, analytical methods and instrumental chemistry, new technologies and new drugs, among others.
Pharmaceutical Sciences Seminar II
Syllabus: Subjects related to the pharmaceutical sciences, relevant to the research lines of bioactive natural and synthetic products, technology and quality control. They should cover topics on bioactive compound chemistry, plant secondary metabolism, pharmacobotany, pharmacology, toxicology, pharmacokinetics, pharmacotechnics, pharmaceutical development and technology, drug quality control, analytical methods and instrumental chemistry, new technologies and new drugs. Presentation of student seminars with topics related to their work.
Bio-organic Drug Synthesis
Syllabus: It aims to study the use of microorganisms (filamentous bacteria and fungi) in the regal and stereoselective synthesis of antibiotics, steroids and alkaloids. It discusses the application of biocatalysts in the synthesis of new drugs, their metabolites and unpublished substances with potential pharmacological activity.
Medicinal Organic Synthesis
Syllabus: To enable the discussion of the theoretical and practical aspects fundamental and necessary for the understanding of the bases of Organic Chemistry, reaction mechanism, stereochemistry, molecular orbital theory, retrosynthetic analysis, rearrangements, applied to biochemical processes, organic and bioorganic drug synthesis.
Syllabus: Experimental Animals. Basic concepts in Toxicology. Types of preclinical pharmacological and toxicological trials (in vivo, ex vivo, in vitro). Preclinical toxicity: acute (DLA, oral LD50, dermal LD50, inhalation LC50), subacute, and chronic toxicity assays; dermal and eye irritation, dermal sensitization; mutagenesis, embryophetotoxicity, carcinogenicity; neurotoxicity; toxicokinetics; extrapolation to humans; use of epidemiological data as toxicity assessment; special toxicity tests (immunotoxicity, hepatotoxicity, nephrotoxicity). Revision of the regulation on Experimental Toxicology in Brazil. International toxicity assessment standards.
Special Topics in Phytochemistry
Syllabus: Main classes of metabolites, general methods of extraction, purification, isolation and notions of structural elucidation of molecules present in natural products of plant origin. Biological activity.
Syllabus: Fundamentals of scientific methodology. Experimental error. Graphical representation of data. Probability and random sampling. Distributions Principles of inference and statistical estimation. Significance tests and curve fitting.
Quality Control of Inputs and Medicines
Syllabus: Provide theoretical support for the evaluation of the quality of pharmaceutical and related products through qualitative and quantitative analysis.
Syllabus: Assembly by students or demonstration by teachers of various preparations commonly used in Pharmacology, with emphasis on the methodologies used in routine laboratories. From the results, pharmacological parameters will be obtained, discussion of the advantages and limitations of each methodology as well as pharmacological and statistical concepts that can be visualized in these experiments.
Microbiological Tests of Medicinal Plants
Syllabus: Evaluation of the microbiological quality of medicinal plants. Methods of determination of antimicrobial activity. Determination of microbial load. Research of bacterial pathogens. Researches contaminant fungi and mycotoxin producers.
Syllabus: Methods of phytochemical investigation. Sources of information on plant species of therapeutic and industrial interest. Biosynthesis of secondary metabolites of plant origin. Chemical taxonomy. Methods of structural determination of organic compounds.
Biological Tests Applied to Natural Products
Syllabus: Popular knowledge about medicinal plants and natural products of the Amazon region: ethnopharmacology. The role of medicinal plants in the history of medicines. Raw material sourcing, stabilization and drying. Processes and quality control of natural extracts. Selection, planning and methodology of trials, preclinical and clinical, with medicinal plants and natural products with biological activity. Biological validation of natural and synthetic products. Toxicity assessment. Natural products used for therapeutic purposes.
Chromatography Applied to Bioactive Product Analyzes
Syllabus: Enable theoretical learning and application of the basic principles of high-performance liquid gas chromatography and validation of chromatographic methods for analysis of synthetic and bioactive natural products.
Extractive Processes and Pharmaceutical Application
Syllabus: Aims to establish basic and applied concepts of obtaining, processing and characterization of plant material; address methods of stabilization and drying of plant material; produce plant extracts; implement extractive operations: maceration, percolation, infusion, decoction, digestion; characterize chemistry and physicochemical of plant extracts; discuss possible applications in pharmacotechnics.